Coding

Part:BBa_K2272015:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pVHb-Cytochrome(Cyt)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 194
    Illegal NgoMIV site found at 242
    Illegal NgoMIV site found at 248
    Illegal NgoMIV site found at 263
    Illegal NgoMIV site found at 997
    Illegal NgoMIV site found at 1069
    Illegal NgoMIV site found at 1117
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1644
    Illegal BsaI.rc site found at 1800


Design Notes

The VHb promoter and the cytochrome coding sequence was ligated with SCAR.


Source

https://parts.igem.org/Part:BBa_K2272000 https://parts.igem.org/Part:BBa_K2272003

References